![]() ![]() Technically, it is a high-quality, high-speed enzyme that digests the DNA sample in 5 to 15 minutes. It cleaves DNA at the site of G/AATTC palindromic sequences which is present after 46 bases. The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot.The restriction enzyme that was used in the analysis is called EcoRV and it recognises and cuts the following sequence GAT/ATC &CTA/TAG If the following sequence was restricted by the above enzyme, how many bands would appear on the gel?It was derived from the E.Coli strain RY13 and hence named EcoRI. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?.Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?.Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?.How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?.Why do I see additional DNA bands on my gel after a restriction digest?. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |